Applications of Real-time PCR in Clinical Microbiology

The introduction of real-time PCR assays to the clinical microbiology laboratory has led to significant improvements in the diagnosis of infectious disease. There has been an explosion of interest in this technique since its introduction and several hundred reports have been published describing applications in clinical bacteriology, parasitology and virology. There are few areas of clinical microbiology which remain unaffected by this new method. It has been particularly useful to detect slow growing or difficult to grow infectious agents. However, its greatest impact is probably its use for the quantitation of target organisms in samples. The ability to monitor the PCR reaction in realtime allows accurate quantitation of target sequence over at least six orders of magnitude. The closed-tube format which removes the need for post-amplification manipulation of the PCR products also reduces the likelihood of amplicon carryover to subsequent reactions reducing the risk of false-positives. As more laboratories begin to utilise these methods standardisation of assay protocols for use in diagnostic clinical Bacterial Gene Regulation and Transcriptional Networks Edited by: M Madan Babu c. 280 pp, January 2013 ISBN: 978-1-908230-14-0, $319/£159 The latest research observations and current theories on transcriptional regulation and gene circuits in bacteria. Two-Component Systems in Bacteria Edited by: R Gross, D Beier c. 410 pp, August 2012 ISBN: 978-1-908230-08-9, $360/£180 Structure-function analysis, sensing mechanisms, atypical two-component systems, stress responses, developmental processes, virulence and symbiosis. 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Sensory Mechanisms in Bacteria: Molecular Aspects of Signal Recognition Edited by: S Spiro, R Dixon x + 268 pp, September 2010 ISBN: 978-1-904455-69-1, $319/£159 An international team of experts reviews a selection of important model systems, providing a timely snapshot of the current state of research in the field. “an excellent volume” (Microbiol. Today) ORDER FROM (UK/Europe): Caister Academic Press, c/o Book Systems Plus, 1st Floor, 8 Hill St., Saffron Walden, Essex, CB10 1JD, UK Tel: 01799 524458 Fax: 01799 524459 http://uk.caister.com ORDER FROM (USA): Caister Academic Press, c/o ISBS, Inc., 920 NE 58th Avenue, Suite 300, Portland OR 97213-3786, USA Tel: 503 287-3093 Fax: 503 280-8832 http://usa.caister.com FULL DETAILS OF ALL OUR BOOKS AT WWW.CAISTER.COM COMING SOON Regulatory Networks Quantitative Real-time PCR in Applied Microbiology Edited by: Martin Filion x + 242 pp, May 2012 ISBN: 978-1-908230-01-0, $319/£159 Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. PCR Troubleshooting and Optimization The Essential Guide Edited by: S Kennedy, N Oswald viii + 236 pp, January 2011 ISBN: 978-1-904455-72-1, $319/£159 Control, optimize and troubleshoot PCR, reverse transcriptase PCR, realtime PCR and quantitative PCR. An essential book. “an essential book ... a valuable tool to all those interested in PCR” (Doodys); “an essential guide” Aus. J. Med. Sci. Real-Time PCR in Food Science Current Technology and Applications Edited by: D Rodriguez-Lazaro c. 280 pp, January 2013 ISBN: 978-1-908230-15-7, $319/£159 An indispensable manual on realtime PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens. Real-Time PCR: Advanced Technologies and Applications Edited by: Nick Saunders and Martin A. Lee An essential manual presenting a comprehensive guide to the most upto-date technologies and applications as well as providing vital insights into the theory of this important technique. ... coming so on! 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